Dr Bikasih Thapa & Dr Maheswar Prasad (Nepal) - Hideo Wada MD PhD (japan) - Dr a Lavra Castrocatesana (Mexico) - Dr Mrs N.M. Hettiarachechui (Srilanka) - Dr Jorge Aldrete Velasco (Mexico) - Prof Hans Peter Kohler (Switzerland) - Dr Hermanus Suhartono S Sp.OG(K) PhD - Dr Isabel Pinheiro (Portugal) - Dr Suranga (Srilanka) - Jovia Dino Jansen Amsterdam,Holand - Hideo Wada MD PhD University Graduate School of Medicine Departement of Moleculer and Laboratory Medicine Japan - DR Bikash Thapa Internal Medicine Nepal University - DR Maheswar Prasad Internal Medicine Nepal University - Dr a Lavra Castro Castresana Colegio de Medicina interna de Mexico - Dr Suransa Manilgama University of Srilanka Internal Departement Medicine - Dr Mrs N.M. Hettiarachechui University of Medicine Srilanka - Dr Jorge Aldrete Velaso .Colegio de Medicina Interna de Mexico - Prof Hans Peter Kholer M.D FACD Profesor of Medicine University ot Switzerland - Dr Ramezan Ali Atace . Baqiyatallah University of Medical Sciences Departement of Micrology Tehran Iran - Ezekiel Wong Toh Yoon Dr. Gastroenterology of Japan - D Eric Beck,MD Bethesda Hospital Capitol Boelevard St Paul USA - Dr Emine Guderen Sahin Istambul University of Internal Medicine Turky - Dr Selmin Toplan Istambul University - Dr Nicholas New Australia - Dr Kughan Govinden. Tropical Infection of Internal Medicine Malaysia - Dr Godfrey M Rwegerera Princes Marina Hospital Bostwana -

Author : Latifah Anandari*, Astuti Giantini


Aim: Procalcitonin is a calcitonin prohormone, its elevation used as one of many sepsis parameters. Procalcitonin Easy Diagnosis is an immunochromatographic cassette, its color intensity shift measured with imaging technology to determine procalcitonin level. Brahms Kryptor is an immunoassay that uses TRACE (Time Resolved Amplified Cryptate Emission) method. This study aims to determine agreement between the two method of measurement. Method: Test for comparison of two method is used. Precision test performed using control cassette with fixed level (25 ng/mL), run 10 times consecutively. Precision test using patient samples performed at low level (< 0.5 ng/mL), high ( > 0.5 ng/mL), and very high ( > 2 ng/mL), each runs 5 times consecutively. Subject of the study settled at 40, based on minimum sample requirement for comparison test. Correlation test and Bland-Altman plot used to analyze data set. P value < 0.05 is considered significant with 95% confidence interval. Results: Coefficient of variant (CV) obtained from control cassette is 2.528 %. Result for CV in low level (0.13 ng/mL), high 0.67 ng/mL), and very high (2.34 ng/mL) are 13.07%, 21.81%, and 13.77% respectively. Spearman correlation resulted r = 0.898 ( p < 0.05). Bland-Altman analysis performed in four groups of data set, < 0.5 ng/mL, > 0.5 ng/mL, > 2 ng/mL, and > 10 ng/mL. In the first group, there are two result outside limits of agreement. Agreement between the two methods is 95 %. Discussion: There is a significant difference between precision test using control cassette and patient samples. Control materials that fit standard requirement is needed. There is a good correlation between two methods. The two value outside limits of agreement in the first group are higher than > 0.2 ng/mL mean, showing a decreased agreement in higher procalcitonin level. Conclusion: There are good correlation and agreement between the two methods. It is advised to provide control material that fit control material requirements. Key words: Procalcitonin, immunochromatographic assay, TRACE, comparison test.

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