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Dr Bikasih Thapa & Dr Maheswar Prasad (Nepal) - Hideo Wada MD PhD (japan) - Dr a Lavra Castrocatesana (Mexico) - Dr Mrs N.M. Hettiarachechui (Srilanka) - Dr Jorge Aldrete Velasco (Mexico) - Prof Hans Peter Kohler (Switzerland) - Dr Hermanus Suhartono S Sp.OG(K) PhD - Dr Isabel Pinheiro (Portugal) - Dr Suranga (Srilanka) - Jovia Dino Jansen Amsterdam,Holand - Hideo Wada MD PhD University Graduate School of Medicine Departement of Moleculer and Laboratory Medicine Japan - DR Bikash Thapa Internal Medicine Nepal University - DR Maheswar Prasad Internal Medicine Nepal University - Dr a Lavra Castro Castresana Colegio de Medicina interna de Mexico - Dr Suransa Manilgama University of Srilanka Internal Departement Medicine - Dr Mrs N.M. Hettiarachechui University of Medicine Srilanka - Dr Jorge Aldrete Velaso .Colegio de Medicina Interna de Mexico - Prof Hans Peter Kholer M.D FACD Profesor of Medicine University ot Switzerland - Dr Ramezan Ali Atace . Baqiyatallah University of Medical Sciences Departement of Micrology Tehran Iran - Ezekiel Wong Toh Yoon Dr. Gastroenterology of Japan - D Eric Beck,MD Bethesda Hospital Capitol Boelevard St Paul USA - Dr Emine Guderen Sahin Istambul University of Internal Medicine Turky - Dr Selmin Toplan Istambul University - Dr Nicholas New Australia - Dr Kughan Govinden. Tropical Infection of Internal Medicine Malaysia - Dr Godfrey M Rwegerera Princes Marina Hospital Bostwana -
ANALYSIS DIVERSITY OF BACTERIA ISOLATED FROM PULMONARY TB PATIENTS WITH 16S rRNA GENE SEQUENCING TECHNIQUE AND TEST RESISTANCES OF ANTIBIOTICS

Author : Ali Awan*

Abstract

Pulmonary infections are the case that frequently happends. Based on the initial survey that conducted at several hospitals in Ambons city, it is found that the presentation of patients who treated with pulmonary TB case, hence, it is needed to take as basis in executing “educated gues therapy”. Isolated and identifications of bacteria is done by morphological observation and molecular technique using genetic information in the small subunit if ribosom 16S Rrna. Furthermore, resistance antimicroba testing by using “ Disc Diffusion Method” according to the National Committee for Clinical Laboratory Standards (NCCLS). Based on the identification result, pathogen bacterial that obtained is “ Streptococcus mitis, which those there kind of bacteries had resisted toward the antibiotics varience of Sulfamethoxaxole, Trimethoprim and Ceftazidime. Key words: Pulmonary infections, pathogen bacterial, Gen sequencing, Antibiotics resistent.

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